- Authors
- Jialong Chen
- Quanli Li
- Jianguang Xu
- Le Zhang
- Manfred F. Maitz
- Jun Li
- title
- Thromboresistant and rapid-endothelialization effects of dopamine and staphylococcal protein A mediated anti-CD34 coating on 316L stainless steel for cardiovascular devices
- Please use the following URL when quoting:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-360672
- original0000000000000
- Journal of Materials Chemistry B : Materials for biology and medicine
Erscheinungsjahr: 2015
Jahrgang: 3
Heft: 13
Seiten: 2615-2623
E-ISSN: 2050-7518 - publication_date
- 2015
- Abstract (EN)
- There is convincing evidence in vivo that the vascular homing of endothelial progenitor cells (EPCs) contributes to rapid endothelial regeneration, which could prevent thrombosis and restenosis of cardiovascular devices. To enhance the EPC homing on cardiovascular devices, immobilization of an EPC capture agent (e.g. an anti-CD34 antibody) on the surface of cardiovascular devices is critical. We describe a way of immobilizing anti-CD34 Ab on 316L Stainless Steel (316L SS). For this, surface modification of 316L SS was performed via self-polymerization of dopamine (DA) and covalent grafting of staphylococcal protein A (SPA). On this coating the anti-CD34 Abs were oriented immobilized through their Fc constant region with SPA. In this process, the results of quartz crystal microbalance, X-ray photoelectron spectroscopy and water contact angle studies indicate that DA, SPA and anti-CD34 Ab were successfully immobilized onto the surface step by step. In vitro blood-compatibility tests confirmed that the modified surface induced less pro-coagulant fibrinogen denaturation, less platelet adhesion and lower activation of the adherent platelets. The affinity of EPCs for the modified surface has been demonstrated under flow conditions. This study provides potential applications for cardiovascular implant materials.
- otherVersion00000
- Link zum Artikel, der zuerst in der Zeitschrift 'Journal of Materials Chemistry B' erschienen ist.
DOI: 10.1039/c4tb01825g - Keywords (DE)
- endotheliale Vorläuferzellen (EPCs), kardiovaskuläre Vorrichtungen, Selbstpolymerisation, kardiovaskuläre Implantatmaterialien
- Keywords (EN)
- endothelial progenitor cells (EPCs), cardiovascular devices, self-polymerization, cardiovascular implant materials
- Classification (DDC)
- 540
- 570
- 610
- Publishing house
- Royal Society of Chemistry, London
- Project sponsoring
- Natural Science Foundation of China
ID: 31300792
China Postdoctoral Science Foundation
ID: 2014M561813
Anhui Provincial Natural Science FundID: 1408085QH149
Anhui Medical UniversityGrants for Scientific Research
ID: XJ201232 - version
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-360672
- Qucosa date of publication
- 07.01.2020
- Document type
- article
- Document language
- English
- licence