- AutorIn
- Sarah Tsurkan
- Titel
- Designer Nuclease-Assisted Targeting to Engineer Mammalian Genomes
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-322907
- Datum der Einreichung
- 16.11.2017
- Datum der Verteidigung
- 28.03.2018
- Abstract (EN)
- Designer nucleases have greatly simplified small genome modifications in many genomes. They can precisely target a specific DNA sequence within a genome and make a double stranded break (DSB). DNA repair mechanisms of the DSB lead to gene mutations or gene modification by homologous directed repair (HDR) if a repair template is exogenously supplied. Thus, small, site directed mutations are easily and quickly achieved. However, strategies that utilize designer nucleases for more complex tasks are emerging and require optimization. To optimize CRISPR/Cas9 assisted targeting, an HPRT rescue assay was utilized to measure the relationship between targeting frequency and homology arm length in targeting constructs in mouse embryonic stem cells. The results show that different gene engineering exercises had different homology requirements.
- Freie Schlagwörter (EN)
- CRISPR/Cas9, Genome Engineering, Targeting Efficiency, Nuclease Assisted Targeting
- Klassifikation (DDC)
- 570
- Klassifikation (RVK)
- WD 5050
- GutachterIn
- Prof. Dr. A. Francis Stewart
- Prof. Dr. Yixin Zhang
- Den akademischen Grad verleihende / prüfende Institution
- Technische Universität Dresden, Dresden
- Sonstige beteiligte Institution
- Biotechnologischen Zentrum (BIOTEC) der Technischen Universität Dresden, Dresden
- Version / Begutachtungsstatus
- aktualisierte Version
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-322907
- Veröffentlichungsdatum Qucosa
- 30.11.2018
- Dokumenttyp
- Dissertation
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis