- Titel
- Fully refractive telecentric f-theta microscope based on adaptive elements for 3D raster scanning of biological tissues
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-954004
- Quellenangabe
- Optics express
Erscheinungsjahr: 2023
Jahrgang: 31
Heft: 18
Seiten: 29703-29715
E-ISSN: 1094-4087 - Erstveröffentlichung
- 2023
- Abstract (EN)
- Various techniques in microscopy are based on point-wise acquisition, which provides advantages in acquiring sectioned images, for example in confocal or two-photon microscopy. The advantages come along with the need to perform three-dimensional scanning, which is often realized by mechanical movement achieved by stage-scanning or piezo-based scanning in the axial direction. Lateral scanning often employs galvo-mirrors, leading to a reflective setup and hence to a folded beam path. In this paper, we introduce a fully refractive microscope capable of three-dimensional scanning, which employs the combination of an adaptive lens, an adaptive prism, and a tailored telecentric f-theta objective. Our results show that this microscope is capable to perform flexible three-dimensional scanning, with low scan-induced aberrations, at a uniform resolution over a large tuning range of X = Y = 6300 µm and Z = 480 µm with only transmissive components. We demonstrate the capabilities at the example of volumetric measurements on the transgenic fluorescence of the thyroid of a zebrafish embryo and mixed pollen grains. This is the first step towards flexible aberration-free volumetric smart microscopy of three-dimensional samples like embryos and organoids, which could be exploited for the demands in both lateral and axial dimensions in biomedical samples without compromising image quality
- Andere Ausgabe
- Link zum Artikel, der zuerst in der Zeitschrift „Optics express” bei Optica Publishing Group erschienen ist.
DOI: 10.1364/OE.496150 - Freie Schlagwörter (DE)
- Biologie, dreidimensionales Scannen, vollrefraktives 3D-Raster-Scanning-Mikroskop, adaptive Linsen
- Freie Schlagwörter (EN)
- biology, three-dimensional scanning, fully refractive 3D raster scanning microscope, adaptive lenses
- Klassifikation (DDC)
- 530
- Verlag
- Optica Publishing Group, Washington, DC
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-954004
- Veröffentlichungsdatum Qucosa
- 05.02.2025
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
CC BY 4.0