- Titel
- Advanced Insights into Catalytic and Structural Features of the Zinc-Dependent Alcohol Dehydrogenase from Thauera aromatica
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-905533
- Quellenangabe
- ChemBioChem
Erscheinungsjahr: 2022
Jahrgang: 23
Heft: 15
E-ISSN: 1439-7633
Artikelnummer: e202200149 - Erstveröffentlichung
- 2022
- Abstract (EN)
- The asymmetric reduction of ketones to chiral hydroxyl compounds by alcohol dehydrogenases (ADHs) is an established strategy for the provision of valuable precursors for fine chemicals and pharmaceutics. However, most ADHs favor linear aliphatic and aromatic carbonyl compounds, and suitable biocatalysts with preference for cyclic ketones and diketones are still scarce. Among the few candidates, the alcohol dehydrogenase from Thauera aromatica (ThaADH) stands out with a high activity for the reduction of the cyclic α-diketone 1,2-cyclohexanedione to the corresponding α-hydroxy ketone. This study elucidates catalytic and structural features of the enzyme. ThaADH showed a remarkable thermal and pH stability as well as stability in the presence of polar solvents. A thorough description of the substrate scope combined with the resolution and description of the crystal structure, demonstrated a strong preference of ThaADH for cyclic α-substituted cyclohexanones, and indicated structural determinants responsible for the unique substrate acceptance.
- Andere Ausgabe
- Link zum Artikel der zuerst in der Zeitschrift „ChemBioChem” bei Wiley erschienen ist.
DOI: 10.1002/cbic.202200149 - Freie Schlagwörter (DE)
- Biokatalyse, Enzymkatalyse, Oxidoreduktasen, Reduktion, Struktur-Wirkungsbeziehungen
- Freie Schlagwörter (EN)
- biocatalysis, enzyme catalysis, oxidoreductases, reduction, structure-activity relationships
- Klassifikation (DDC)
- 540
- Verlag
- Wiley, Weinheim
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-905533
- Veröffentlichungsdatum Qucosa
- 08.04.2024
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
CC BY 4.0