- Titel
- Glycosaminoglycans and their sulfate derivatives differentially regulate the viability and gene expression of osteocyte-like cell lines
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-356895
- Quellenangabe
- Journal of bioactive and compatible polymers Erscheinungsort: Thousand Oaks
Verlag: Sage
Erscheinungsjahr: 2014
Jahrgang: 29
Heft: 5
Seiten: 474-485
E-ISSN: 1530-8030 - Erstveröffentlichung
- 2014
- Abstract (EN)
- Collagen and glycosaminoglycans, such as hyaluronan and chondroitin sulfate, are the major components of bone extracellular matrix, and extracellular matrix composites are being evaluated for a wide range of clinical applications. The molecular and cellular effects of native and sulfatemodified glycosaminoglycans on osteocytes were investigated as critical regulators of bone remodeling. The effects of glycosaminoglycans on viability, necrosis, apoptosis, and regulation of gene expression were tested in two osteocyte-like cell lines, the murine MLO-Y4 and the rat UMR 106-01 cells. Glycosaminoglycans were non-toxic and incorporated by osteocytic cells. In MLO-Y4 cells, sulfation of glycosaminoglycans led to a significant inhibition of osteocyte apoptosis, 42% inhibition for highly sulfated chondroitin sulfate and 58% for highly sulfated hyaluronan, respectively. Cell proliferation was not affected. While treatment with highly sulfated chondroitin sulfate increased cell viability by 20% compared to the native chondroitin sulfate. In UMR 106- 01 cells, treatment with highly sulfated hyaluronan reduced the receptor activator of nuclear factor-κB ligand/osteoprotegerin ratio by 58% compared to the non-sulfated form, whereas highly sulfated chondroitin sulfate led to 60% reduction in the receptor activator of nuclear factor-κB ligand/osteoprotegerin ratio in comparison to the native chondroitin sulfate. The expression of SOST, the gene encoding sclerostin, was reduced by 50% and 45% by highly sulfated hyaluronan and chondroitin sulfate, respectively, compared to their native forms. The expression of BMP- 2, a marker of osteoblast differentiation, was doubled after treatment with the highly sulfated hyaluronan in comparison to its native form. In conclusion, highly sulfated glycosaminoglycans inhibit osteocyte apoptosis in vitro and promote an osteoblast-supporting gene expression profile.
- Andere Ausgabe
- Link zum Artikel, der zuerst in der Zeitschrift 'Journal of bioactive and compatible polymers' erschienen ist.
DOI: 10.1177/0883911514546983 - Freie Schlagwörter (DE)
- Extrazelluläre Matrix, Knochenumbau, Chondroitinsulfat, Hyaluronan, Osteozyten, Genübertragung, Genkodierung für sklerostin, sulfatierte Glykosaminoglykane
- Freie Schlagwörter (EN)
- Extracellular matrix, bone remodeling, chondroitin sulfate, hyaluronan, osteocyte, gene delivery, gene encoding sclerostin, sulfated glycosaminoglycans, apoptosis, osteoprotegerin
- Klassifikation (DDC)
- 540, 610
- Verlag
- Sage, London
- Förder- / Projektangaben
- Deutsche Forschungsgemeinschaft Transregio 67
subproject A2, A3, and B2 - Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-356895
- Veröffentlichungsdatum Qucosa
- 11.10.2019
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
