- Titel
- Inflammation-related alterations of lipids after spinal cord injury revealed by Raman spectroscopy
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-352900
- Quellenangabe
- Journal of Biomedical Optics Herausgeber: Brian W. Pogue
Erscheinungsort: Bellingham, Wash.
Verlag: SPIE
Erscheinungsjahr: 2016
Jahrgang: 21
Heft: 6
Seiten: 061008-1-061008-8
ISSN: 1560-2281 - Erstveröffentlichung
- 2016
- Abstract (EN)
- Spinal cord injury (SCI) triggers several lipid alterations in nervous tissue. It is characterized by extensive demyelination and the inflammatory response leads to accumulation of activated microglia/macrophages, which often transform into foam cells by accumulation of lipid droplets after engulfment of the damaged myelin sheaths. Using an experimental rat model, Raman microspectroscopy was applied to retrieve the modifications of the lipid distribution following SCI. Coherent anti-Stokes Raman scattering (CARS) and endogenous two-photon fluorescence (TPEF) microscopies were used for the detection of lipid-laden inflammatory cells. The Raman mapping of CH2 deformation mode intensity at 1440 cm−1 retrieved the lipid-depleted injury core. Preserved white matter and inflammatory regions with myelin fragmentation and foam cells were localized by specifically addressing the distribution of esterified lipids, i.e., by mapping the intensity of the carbonyl Raman band at 1743 cm−1, and were in agreement with CARS/TPEF microscopy. Principal component analysis revealed that the inflammatory regions are notably rich in saturated fatty acids. Therefore, Raman spectroscopy enabled to specifically detect inflammation after SCI and myelin degradation products.
- Andere Ausgabe
- Link zum Artikel, der zuerst in der Zeitschrift Journal of Biomedical Optics' erschienen ist.
DOI: 10.1117/1.JBO.21.6.061008 - Freie Schlagwörter (DE)
- Ramanspektroskopie, Multiphotonenprozesse, Gewebe, biomedizinische Optik
- Freie Schlagwörter (EN)
- Raman spectroscopy, multiphoton processes, tissues, biomedical optics
- Klassifikation (DDC)
- 530, 610
- Verlag
- SPIE, Bellingham, Wash.
- Förder- / Projektangaben
- Bundesministerium für Wirtschaft und Technologie MediCARS project
ID: 13N10777 - Technischen Universität Dresden, Medizinische Fakultät Habilitationsförderung für Frauen
- Forschungszentrum für regenerative Therapien
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-352900
- Veröffentlichungsdatum Qucosa
- 09.09.2019
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
