- Titel
- Holographically generated structured illumination for cell stimulation in optogenetics
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-349767
- Konferenz
- SPIE Digital Optical Technologies. Munich, 25.-29. June 2017
- Quellenangabe
- Digital Optical Technologies 2017
Herausgeber: Bernard C. Kress
Herausgeber: Wolfgang Osten
Herausgeber: H. Paul Urbach
Erscheinungsort: Bellingham, Wash.
Verlag: SPIE
Erscheinungsjahr: 2017
Titel Schriftenreihe: Proceedings of SPIE
Bandnummer Schriftenreihe: 10335 - Erstveröffentlichung
- 2017
- Abstract (EN)
- In Optogenetics, cells, e.g. neurons or cardiac cells, are genetically altered to produce for example the lightsensitive protein Channelrhodopsin-2. Illuminating these cells induces action potentials or contractions and therefore allows to control electrical activity. Thus, light-induced cell stimulation can be used to gain insight to various biological processes. Many optogenetics studies, however, use only full field illumination and thus gain no local information about their specimen. But using modern spatial light modulators (SLM) in conjunction with computer-generated holograms (CGH), cells may be stimulated locally, thus enabling the research of the foundations of cell networks and cell communications. In our contribution, we present a digital holographic system for the patterned, spatially resolved stimulation of cell networks. We employ a fast ferroelectric liquid crystal on silicon SLM to display CGH at up to 1.7 kHz. With an effective working distance of 33 mm, we achieve a focus of 10 µm at a positioning accuracy of the individual foci of about 8 µm. We utilized our setup for the optogenetic stimulation of clusters of cardiac cells derived from induced pluripotent stem cells and were able to observe contractions correlated to both temporal frequency and spatial power distribution of the light incident on the cell clusters.
- Andere Ausgabe
- Link zum Artikel, der zuerst in der Zeitschrift 'Proceedings of SPIE' erschienen ist.
DOI: 10.1117/12.2270281 - Freie Schlagwörter (DE)
- Optogenetik, räumlicher Lichtmodulator, computergeneriertes Hologramm
- Freie Schlagwörter (EN)
- optogenetics, spatial light modulator, computer-generated hologram
- Klassifikation (DDC)
- 620
- Verlag
- SPIE, Bellingham, Wash.
- Förder- / Projektangaben
- Deutsche Forschungsgemeinschaft ID: Cz55-30
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-349767
- Veröffentlichungsdatum Qucosa
- 13.08.2019
- Dokumenttyp
- Konferenzbeitrag
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
