- Titel
- Evaluation of Pulmonary Edema: Stereological versus Gravimetrical Analysis
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa-132491
- Quellenangabe
- Eur Surg Res 2001;33:270–278, ISSN: 0014-312X
- Erstveröffentlichung
- 2001
- Abstract (DE)
- Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
- Abstract (EN)
- Assessment of lung edema by gravimetrical analysis is a standard method to evaluate the severity of experimentally induced ischemia/reperfusion (IR) injury. The aim of this study was to compare gravimetrical assessment of pulmonary edema with a stereological approach which allows for qualitative and quantitative distinction between intravascular and edematous fluids by light microscopy. Eight experimental groups which differed in mode of preservation, ischemic storage and pharmacological treatments were studied in an extracorporeal rat lung model. Analysis of the pooled data showed that the wet/dry ratio values mainly reflected the amount of intra-alveolar edema (rs = 0.442; p = 0.0057) but only stereological assessment of edema formation revealed differences depending on the treatment used. Only stereological data correlated significantly with oxygen tension measured at the end of reperfusion (rs = –0.530; p = 0.0009). We conclude that gravimetry is of minor functional importance compared to assessment by stereological methods which prove to be a reliable and efficient tool for the evaluation of IR injury in the different experimental settings.
- Andere Ausgabe
- DOI: 10.1159/000049717
- Volltext des Artikels, der zuerst in der Zeitschrift 'European Surgical Research' des Karger-Verlages erschienen ist.
Link: http://dx.doi.org/10.1159/000049717 - Freie Schlagwörter (DE)
- Lunge, Reperfusionsschaden, Ischämie, Transplantation
- Freie Schlagwörter (EN)
- Lung, Ischemia reperfusion injury, Wet-to-dry weight ratio, Transplantation
- Klassifikation (DDC)
- 610
- Klassifikation (RVK)
- XA 10000
- Verlag
- Karger, Basel
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa-132491
- Veröffentlichungsdatum Qucosa
- 12.02.2014
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
