- Titel
- Characterisation of the core microbiome of anaerobic digestion systems and the potential of manipulating the microbiome by biostimulation and bioaugmentation.
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-1015160
- Übersetzter Titel (DE)
- Charakterisierung des Kernmikrobioms anaerober Vergärungssysteme und das Potenzial der Manipulation des Mikrobioms durch Biostimulation und Bioaugmentation.
- Erstveröffentlichung
- 2026
- Datum der Einreichung
- 11.09.2025
- Datum der Verteidigung
- 15.12.2025
- Abstract (EN)
- This dissertation provides future-oriented insights into the microbiome of anaerobic digestion systems and explores novel concepts of biostimulation utilising active gases such as O2 and H2 as biostimulants. The dissertation is structured into four sections, each corresponding to one of the authored articles. Before presenting these articles, the theoretical foundations and contextual framework are outlined. The initial chapters provide an introduction to the fundamentals of anaerobic digestion, its technical implementation and innovative treatment technologies. The concept of biomanipulation is then explained in more detail, with a focus on the two pillars of biostimulation and bioaugmentation. Finally, the methods used for sampling, as well as the measurement of operational, chemical and taxonomic parameters, bioinformatic and multivariate approaches and fuzzy logic control are described. In the first article, the microbiomes of 80 industrial anaerobic digestion systems were analysed through a holistic investigation of operational, chemical and taxonomic data. These were statistically evaluated, and depending on whether taxonomic, chemical or operational parameters were used, different microbial clusters emerged. In addition, a core microbiome consisting of MBA03, Proteiniphilum, Caldicoprobacter, Methanosarcina, and representatives of the Dethiobacteraceae was identified. The second article expands upon these findings and characterises MBA03 as a representative of the new order Darwinibacteriales for the first time. The third article investigated a patented process for sewage sludge treatment using an O2 reactivation stage on an industrial scale. This process increased biogas yield by up to 55 %, reduced the volume of digestate by 25 %, and removed over 90 % of NH4-N. Microbiological analyses revealed the presence of ammonium-oxidising bacteria, in particular Chitinophagaceae and Candidatus nitrosoglobus. In summary, this O2 activation led to an increase in energy production and a reduction in nitrogenous components and pathogens in the sewage sludge. The fourth article investigated the use of fuzzy logic control for demand-driven H2 supply to regulate biological in-situ methanation. The gradual increase in H2 content based on real-time gas data led to a 49 % increase in CH4 concentration from 58.4 % to 87.0 %. The microbial adaptation favoured hydrogenotrophic methanogens and syntrophic microorganisms, which promoted methanogenesis. The approach represents a stable, efficient control strategy for bio-CH4 production.
- Verweis
- Link: https://doi.org/10.1186/s13068-024-02525-1
Multivariate comparison of taxonomic, chemical and operational data from 80 different full-scale anaerobic digester-related systems
DOI: 10.1186/s13068-024-02525-1 - Unveiling the ecology, taxonomy and metabolic capabilities of MBA03, a potential key player in anaerobic digestion
Link: https://doi.org/10.1101/2023.09.08.556800
DOI: 10.1101/2023.09.08.556800 - Microbiome Characterization after Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge
Link: https://doi.org/10.3390/fermentation9050471
DOI: 10.3390/fermentation9050471 - Adaptation of the anaerobic microbiome for in-situ power-to-methane processes through fuzzy logic control of H2 input
DOI: 10.1016/j.biteb.2025.102194
Link: https://doi.org/10.1016/j.biteb.2025.102194 - Freie Schlagwörter (EN)
- Anaerobic Digestion, Microbiome, Biostimulation, 16s rRNA Sequencing, Microbiology
- Klassifikation (DDC)
- 363
- Klassifikation (RVK)
- AR 21560
- GutachterIn
- Prof. Dr. Christina Dornack
- Prof. Dr. Christian Abendroth
- Prof. Dr. Michael Nelles
- Den akademischen Grad verleihende / prüfende Institution
- Technische Universität Dresden, Dresden
- Förder- / Projektangaben
- EUROPEAN RESEARCH EXECUTIVE AGENCY HORIZON 2020
Natural and Synthetic Microbial Communities for Sustainable Production of Optimised Biogas
(Micro4Biogas)
ID: 101000470 - Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-1015160
- Veröffentlichungsdatum Qucosa
- 26.02.2026
- Dokumenttyp
- Dissertation
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
CC BY 4.0- Nutzungshinweis
- Inhalte mit unterschiedlichem Rechtestatus
- Inhaltsverzeichnis
Table of Contents Danksagung ii Zusammenfassung iv Abstract v Publications vi Table of Contents viii List of Figures x Abbreviations xiv 1 Introduction 1 1.1 Motivation 1 2 Anaerobic Digestion Processes 3 2.2 Basics of Anaerobic Digestion 3 2.3 Technical Application of Anaerobic Digestion 7 2.3.1 Anaerobic Digestion in the European Union 8 2.3.2 Reactor Types 10 2.3.2 Potential Upgrade Technologies for Anaerobic Digestion Systems 13 2.4 Biomanipulation 18 2.4.1 Biostimulation 19 2.4.2 Bioaugmentation 25 2.5 Molecular Biological Approaches 28 2.6 Objective of the work 30 3 Materials & Methods 32 3.1 Sampling Anaerobic Digestion Microbiomes 32 3.2 Operational Parameters 33 3.3 Chemical Parameters 35 3.4 Taxonomic Parameters 38 3.5 Bioinformatic Approaches 44 3.5.1 QIIME 2 44 3.5.2 Alpha Diversity 45 3.5.3 Beta Diversity 46 3.6 Multivariate Approaches 47 3.7 Fuzzy Logic Control 48 4 Research Results 49 4.1 Investigation of the core microbiome and classification of Darwinibacteriales 50 4.1.1 Article 1: Multivariate comparison of taxonomic, chemical and operational data from 80 different full-scale anaerobic digester-related systems 50 4.1.2 Article 2: Unveiling the ecology, taxonomy and metabolic capabilities of MBA03, a potential key player in anaerobic digestion 73 4.2 The investigation of biostimulation through the injection of O2 and H2 92 4.2.1 Article 3: Microbiome Characterisation after Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge 92 4.2.2 Article 4: Adaptation of the anaerobic microbiome for in-situ power-to-CH4 processes through fuzzy logic control of H2 input 112 5 Summary & Conclusion 134 6 Future Research 137 7 References 138 Declarations A Declaration of conformity B