- Titel
- EMT induces characteristic changes of Rho GTPases and downstream effectors with a mitosis-specific twist
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-1009011
- Quellenangabe
- Physical biology
Erscheinungsjahr: 2023
Jahrgang: 20
Heft: 6
E-ISSN: 1478-3975
Artikelnummer: 066001 - Erstveröffentlichung
- 2023
- Abstract (EN)
- Epithelial-mesenchymal transition (EMT) is a key cellular transformation for many physiological and pathological processes ranging from cancer over wound healing to embryogenesis. Changes in cell migration, cell morphology and cellular contractility were identified as hallmarks of EMT. These cellular properties are known to be tightly regulated by the actin cytoskeleton. EMT-induced changes of actin-cytoskeletal regulation were demonstrated by previous reports of changes of actin cortex mechanics in conjunction with modifications of cortex-associated f-actin and myosin. However, at the current state, the changes of upstream actomyosin signaling that lead to corresponding mechanical and compositional changes of the cortex are not well understood. In this work, we show in breast epithelial cancer cells MCF-7 that EMT results in characteristic changes of the cortical association of Rho-GTPases Rac1, RhoA and RhoC and downstream actin regulators cofilin, mDia1 and Arp2/3. In the light of our findings, we propose that EMT-induced changes in cortical mechanics rely on two hitherto unappreciated signaling paths—i) an interaction between Rac1 and RhoC and ii) an inhibitory effect of Arp2/3 activity on cortical association of myosin II.
- Andere Ausgabe
- Link zum Artikel, der zuerst in der Zeitschrift „Physical Biology” im Verlag IOP Publishing erschienen ist.
DOI: 10.1088/1478-3975/acf5bd - Freie Schlagwörter (EN)
- epithelial-mesenchymal transition, actin cortex, cell mechanics, Rho GTPases, atomic force microscopy
- Klassifikation (DDC)
- 530
- 900
- Verlag
- IOP Publishing, Bristol
- Förder- / Projektangaben
- Deutsche Forschungsgemeinschaft (DFG)
Exzellenzcluster (ExStra)
EXC 2068: Physik des Lebens - Die dynamische Organisation lebender Materie
(PoL)
ID: 390729961 - Deutsche Forschungsgemeinschaft (DFG)
Heisenberg-Förderung
Aktive Mechanik lebender Materie: physikalische Konzepte und biologische Funktion
ID: 495224622 - Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:14-qucosa2-1009011
- Veröffentlichungsdatum Qucosa
- 10.12.2025
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
CC BY 4.0